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cf-1 mef feeder layers  (GlobalStem)

 
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    GlobalStem cf-1 mef feeder layers
    Cf 1 Mef Feeder Layers, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cf-1+mef+feeder+layers/irradiated+mouse+embryonic+fibroblast+feeder+cells+cf1+mef/pmc04811722-70-23-22
    Average 90 stars, based on 1 article reviews
    cf-1 mef feeder layers - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Encapsulation:

    Article Title: Microfluidic fabrication of bioactive microgels for rapid formation and enhanced differentiation of stem cell spheroids
    Article Snippet: In brief, recombinant FGF-2, Activin A, and Nodal (1 ng/mL) were added to DMEM containing a 1:6 volume fraction of microgel droplets (120 μm, 7% w/v) and incubated at RT for 16 h. Supernatant was then collected and analyzed by ELISA (R&D [Activin A and FGF]); MyBioSource [Nodal])(n=3), wherein remaining GF concentration was measured and normalized to control samples prepared in DMEM. .. 2.6 mESC encapsulation, differentiation and analysis 2.6.1 mESC culture D3 mouse ESCs (ATCC) were propagated on irradiated mouse embryonic fibroblast (CF-1 MEF, GlobalStem) feeder layers in self-renewal medium consisting of mESC Basal media (ATCC) supplemented with 15% ES-qualified FBS, 1000 U/mL leukemia inhibitory factor (LIF), and 100 nM 2-mercaptoethanol at 37°C, 5% CO 2 and 90–95% humidity, with medium change every day. mESCs were subcultured every 2–3 days onto fresh feeders using trypsin-EDTA. .. 2.6.2 Cell encapsulation experiments mESCs were suspended at a concentration of 25 (10 6 ) cells · mL −1 in a prepolymer solution composed of 8 % w/v 8-arm PEGSH in media supplemented with 20% v/v Optiprep density gradient medium (Sigma), and mixed on-chip with 7% w/v PEGDA and 3% w/v heparin-MA precursor solution.

    Irradiation:

    Article Title: Microfluidic fabrication of bioactive microgels for rapid formation and enhanced differentiation of stem cell spheroids
    Article Snippet: In brief, recombinant FGF-2, Activin A, and Nodal (1 ng/mL) were added to DMEM containing a 1:6 volume fraction of microgel droplets (120 μm, 7% w/v) and incubated at RT for 16 h. Supernatant was then collected and analyzed by ELISA (R&D [Activin A and FGF]); MyBioSource [Nodal])(n=3), wherein remaining GF concentration was measured and normalized to control samples prepared in DMEM. .. 2.6 mESC encapsulation, differentiation and analysis 2.6.1 mESC culture D3 mouse ESCs (ATCC) were propagated on irradiated mouse embryonic fibroblast (CF-1 MEF, GlobalStem) feeder layers in self-renewal medium consisting of mESC Basal media (ATCC) supplemented with 15% ES-qualified FBS, 1000 U/mL leukemia inhibitory factor (LIF), and 100 nM 2-mercaptoethanol at 37°C, 5% CO 2 and 90–95% humidity, with medium change every day. mESCs were subcultured every 2–3 days onto fresh feeders using trypsin-EDTA. .. 2.6.2 Cell encapsulation experiments mESCs were suspended at a concentration of 25 (10 6 ) cells · mL −1 in a prepolymer solution composed of 8 % w/v 8-arm PEGSH in media supplemented with 20% v/v Optiprep density gradient medium (Sigma), and mixed on-chip with 7% w/v PEGDA and 3% w/v heparin-MA precursor solution.



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    Generation of samples for hESC-conditioned Matrigel™ analysis. A, untreated Matrigel™ samples were generated by <t>plating</t> <t>hESCs</t> on a Matrigel™ layer and harvesting after 4 days of growth in <t>MEF-CM.</t> B, control Matrigel™ samples were generated by treating a Matrigel™ layer with cell recovery solution (see Experimental Procedures) and plating hESCs on this layer. hESCs were recovered from the treated layer after 4 days of growth in EB medium. C, conditioned Matrigel™ samples were produced by plating hESCs on a Matrigel™ layer and culturing for 4 to 5 days in MEF-CM. hESCs were subsequently removed from this layer using cell recovery solution to produce conditioned Matrigel™. Fresh hESCs were plated on the conditioned Matrigel™ layer and grown for 4 days in EB medium.
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    Generation of samples for hESC-conditioned Matrigel™ analysis. A, untreated Matrigel™ samples were generated by plating hESCs on a Matrigel™ layer and harvesting after 4 days of growth in MEF-CM. B, control Matrigel™ samples were generated by treating a Matrigel™ layer with cell recovery solution (see Experimental Procedures) and plating hESCs on this layer. hESCs were recovered from the treated layer after 4 days of growth in EB medium. C, conditioned Matrigel™ samples were produced by plating hESCs on a Matrigel™ layer and culturing for 4 to 5 days in MEF-CM. hESCs were subsequently removed from this layer using cell recovery solution to produce conditioned Matrigel™. Fresh hESCs were plated on the conditioned Matrigel™ layer and grown for 4 days in EB medium.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Mass Spectrometry–based Proteomic Analysis of the Matrix Microenvironment in Pluripotent Stem Cell Culture *

    doi: 10.1074/mcp.M112.020057

    Figure Lengend Snippet: Generation of samples for hESC-conditioned Matrigel™ analysis. A, untreated Matrigel™ samples were generated by plating hESCs on a Matrigel™ layer and harvesting after 4 days of growth in MEF-CM. B, control Matrigel™ samples were generated by treating a Matrigel™ layer with cell recovery solution (see Experimental Procedures) and plating hESCs on this layer. hESCs were recovered from the treated layer after 4 days of growth in EB medium. C, conditioned Matrigel™ samples were produced by plating hESCs on a Matrigel™ layer and culturing for 4 to 5 days in MEF-CM. hESCs were subsequently removed from this layer using cell recovery solution to produce conditioned Matrigel™. Fresh hESCs were plated on the conditioned Matrigel™ layer and grown for 4 days in EB medium.

    Article Snippet: H9 (passage 26) and CA1 (passage 20) hESCs and BJ-1D (passage 69) hiPSCs were maintained on CF-1 irradiated MEF feeder layers (GlobalStem, Rockville, MD) using media composed of knockout DMEM/F12, 20% knockout serum replacement, 1% non-essential amino acids, 2 m m glutamine (CellGro, Manassas, VA), 0.1 m m 2-mercaptoethanol (Fisher, Toronto, ON, Canada), and 4 ng/ml of basic fibroblast growth factor (bFGF).

    Techniques: Generated, Control, Cell Recovery, Produced